Bovine Milk Fat Globule Epidermal Growth Factor VIII activates PI3K/Akt signaling pathway and attenuates sarcopenia in rat model induced by d-galactose

To develop a more effective and safer treatment for sarcopenia, this research investigated the anti-sarcopenia mechanism of Milk Fat Globule Epidermal Growth Factor Ⅷ (MFG-E8) from liver function metabolism in sarcopenic model rat. After 4 weeks nutritional intervention experiment, MFG-E8 can significantly increase gastrocnemius mass The improving sarcopenia was related to its promotional capacity activities superoxide dismutase (SOD) activity serum, Glutamic-oxaloacetic transaminase (GOT) glutamic-pyruvic (GPT) liver. Meanwhile, could also down-regulate obesity-related indicators, such as triglyceride (TG) Non-esterified fatty acid (NEFA). analysis histopathology found that reduce accumulation vesicles, improve function, thereby alleviating tissue inflammation. In vitro experiments, myoblasts obtained showed mitochondrial autophagy inhibit cell apoptosis. addition, up-regulate phosphorylation level PI3K via activating PI3K/Akt signaling pathway tissue, promote formation muscle fibers, increasing mass. Moreover, neuromuscular junctions by up-regulating mRNA protein expression MusK gastrocnemius.